![]() If gB and gH/gL must interact for fusion, then these molecules must reach across the space between apposing cells. We believe that this is the first description of a multicomponent viral fusion machine that can be split between cells. Importantly, expression of gB and gH/gL in trans (gB-expressing cells mixed with other gH/gL-expressing cells) resulted in substantial fusion. Coimmunoprecipitation indicated that HCMV gB and gH/gL can interact. We concluded that the HCMV core fusion machinery consists of gB and gH/gL. Coexpression of UL128, UL130, and UL131 did not enhance fusion. Fusion frequently involved the majority of cells, and gB and gH/gL were both necessary and sufficient for fusion, whereas no fusion occurred when either glycoprotein was omitted. In this report, we describe the fusion of epithelial, endothelial, microglial, and fibroblast cells in which HCMV gB and gH/gL were expressed from nonreplicating adenovirus vectors. For other herpesviruses, studies of cell-cell fusion induced by viral proteins have provided substantial information about late stages of entry. However, very little is known about which HCMV glycoproteins mediate entry into various cell types, including relevant epithelial and endothelial cells. ![]() Human cytomegalovirus (HCMV) has the ability to infect a wide variety of human cell types in vivo. ![]() In many cases, this involves binding to abundant glycosaminoglycans or integrins followed by interactions with more limited cell surface proteins, leading to fusion with cellular membranes. ![]() Herpesviruses use a cascade of interactions with different cell surface molecules to gain entry into cells. ![]()
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